- Видео 21
- Просмотров 112 889
Polyplus
Франция
Добавлен 8 мар 2011
Polyplus is a leading upstream solutions provider for advanced biologic and cell and gene therapy production from research to commercial scale. An innovator in nucleic acid delivery, the legacy portfolio features process-centric transfection reagents, kits, and support services. Custom plasmid vector design was integrated into the offer in 2022 as a first measure to expanding the products and services portfolio to help customers (or the industry) optimize process economics while meeting strict scientific and regulatory standards. Headquartered in Europe, the Polyplus team continues to grow globally with operations in the United States and Asia. Learn more at www.polyplus-transfection.com.
Видео
pPLUS® AAV Helper
Просмотров 1119 месяцев назад
pPLUS® AAV-Helper is a novel plasmid helper designed for AAV production. This plasmid is the outcome of an extensive R&D program based on our proprietary DNA assembly technology (e-Zyvec® technology) aiming at exploring the synergies of multiple genetic features to improve the infectivity (measured in TU/mL) and quality (full/empty ratio) of viral particles obtained through triple transfection ...
Polyplus
Просмотров 629 месяцев назад
Polyplus is a leading upstream solutions provider for advanced biologic and cell and gene therapy production from research to commercial scale. An innovator in nucleic acid delivery, the legacy portfolio features process-centric transfection reagents, kits, and support services for viral and non-viral delivery. Custom plasmid vector design and plasmid and protein manufacturing was integrated in...
Transient protein production with FectoCHO Expression System - Polyplus
Просмотров 232Год назад
FectoCHO® Expression System is a 3 component kit optimized to work together: - FectoCHO® CD Expression Medium, a chemically defined cell culture medium optimized for transient gene expression - FectoPRO® Transfection Reagent to produce amazing protein yields using low DNA amount - FectoPRO® Expression Booster that enhances gene expression to boost the protein production 00:00 - Transient protei...
Timelapse Vectura construction 2021
Просмотров 4602 года назад
The moving in the new building is done in two times: support functions (marketing, sales, finance etc) moved in the new building in July and the laboratories employees will move in this fall. This new building will allow us to continue to cater for the exponential growth in demand for raw materials for ATMP development and manufacturing. Indeed, we will be able to meet the expectations of the g...
We are Polyplus®, we enhance transfection
Просмотров 3,7 тыс.4 года назад
Polyplus-transfection® is the leading biotechnology company that supports Gene and Cell therapy, biologics manufacturing and life science research with innovative nucleic acid transfection solutions. Polyplus-transfection®’s strengths are 20 years of experience in manufacturing transfection solutions with tailored scientific and regulatory support to accelerate research. Based on the Science Pa...
![[Webinar] Advances in gene therapy : next-generation transfection reagent for AAV production](http://i.ytimg.com/vi/g8cTgx-rg44/mqdefault.jpg)
[Webinar] Advances in gene therapy : next-generation transfection reagent for AAV production
Просмотров 8724 года назад
The number of ATMP therapeutic-based medicines for inherited genetic disorders is in constant growth, with a global 32% increase in new clinical trials in the last 4 years. ATMPs have demonstrated their success with already more than ten approved for commercialization. Attendees will learn: - How to improve AAV viral vector yields by focusing on transfection step. - How to perform transfection ...
![[Webinar] in vivo delivery from study to gene therapy](http://i.ytimg.com/vi/jIaEVkoIDDk/mqdefault.jpg)
[Webinar] in vivo delivery from study to gene therapy
Просмотров 1,5 тыс.5 лет назад
0:29 in vivo nucleic acid delivery: 1:10 Why? 2:35 How? 4:40 What? 5:52 in vivo jetPEI®: easy, injectable, safe 9:40 in vivo delivery case studies: 9:50 AnxA2 function study 11:37 HIV-infected CNS attenuation 12:50 in vivo for gene therapy 13:15 in vivo jetPEI® Product Range: from R&D to Clinical Trials 15:42 Toolbox: transfection database, scientific materials 16:43 CONTACT US! Check out our n...
![[Webinar] Process development to simplify manufacturing scale up of therapeutic viral vectors](/img/1.gif)
[Webinar] Process development to simplify manufacturing scale up of therapeutic viral vectors
Просмотров 7 тыс.5 лет назад
0:19 Introduction to the webinar 2:10 Presentation of Polyplus-transfection 2:57 Transfection product lines 3:56 Viral Vectors Manufacturing Roadmap 6:59 Current Upstream Manufacturing Challenges 8:15 Optimized PEI for Viral Vector Production: PEIpro® 12:02 Scaling-up systems: adherent and suspension 14:00 Scalable transient transfection: cell culture medium 18:35 Production scale-up 21:20 PEIp...
What is transfection? - Polyplus transfection
Просмотров 65 тыс.5 лет назад
Tutorial provided to you by Transfection Experts. 0:06 Introduction to Transfection 0:34 How to deliver nucleic acids into the cells 2:02 Chemical based DNA transfection mechanism 2:57 What are the applications of transfection 3:54 Visit our website & contact us Transfection is used to study gene expression and protein function by introducing foreign genetic material into a cell. In this video ...
[Webinar] CRISPR-Cas9: overview, challenges and latest technologies - Polyplus transfection
Просмотров 1,5 тыс.5 лет назад
0:40 How does CRISPR-Cas9 work? 2:35 A standard experiment 4:35 Challenges in genome editing 5:03 Choosing the right approach (plasmid, mRNA, protein) 6:35 Plasmid DNA 8:13 RNA - case study 11:52 RNP - case study 15:20 Is cas9 the only one? 16:18 Tips & tricks for genome editing optimization 19:45 CRISPR Toolbox (Polyplus products) 20:15 in-vivo genome editing Check out this new webinar made by...
mRNA transfection tutorial using jetMESSENGER transfection reagent - Polyplus-transfection
Просмотров 2 тыс.5 лет назад
jetMESSENGER® is a highly efficient and gentle mRNA transfection reagent. mRNA transfection presents many advantages versus DNA transfection: - no risk of genome integration, hence no genome modification of the transfected cell - no promoter regulation issue - no need to reach the nucleus for efficient expression more gentle process jetMESSENGER® has been specifically designed to offer outstand...
Easy DNA transfection with jetPRIME - Polyplus-transfection
Просмотров 6 тыс.5 лет назад
jetPRIME® is a powerful and versatile DNA and siRNA transfection reagent for day-to-day experiments that leads to efficient and reliable scientific results. jetPRIME® ensures high DNA transfection efficiency and excellent gene silencing in a variety of adherent cells. jetPRIME ® is also ideal for DNA/siRNA co-transfection or co-delivery of several plasmids. Furthermore, our jetPRIME® reagent is...
Straightforward RNP genome editing with jetCRISPR and SpCas9 Nuclease - Polyplus-transfection
Просмотров 6575 лет назад
jetCRISPR® is an innovative reagent especially designed to deliver RNP in a CRISPR/Cas9 and CRISPR/Cpf1 experiments. jetCRISPR™ leads to high CRISPR genome editing efficiency, while maintaining excellent cell viability and morphology post-transfection. Use the leading technology for CRISPR/Cas9 and CRISPR/Cpf1 RNP delivery! For more information, visit: www.polyplus-transfection.com/products/jet...
Transient protein production with FectoCHO Expression System - Polyplus-transfection
Просмотров 1,2 тыс.5 лет назад
FectoCHO® Expression System is a 3 component kit optimized to work together: - FectoCHO® CD Expression Medium, a chemically defined cell culture medium optimized for transient gene expression - FectoPRO® Transfection Reagent to produce amazing protein yields using low DNA amount - FectoPRO® Expression Booster that enhances gene expression to boost the protein production 00:00 - Transient protei...
Transient transfection for Lentivirus & AAV production - BPI Interview - Polyplus transfection
Просмотров 1,5 тыс.5 лет назад
Transient transfection for Lentivirus & AAV production - BPI Interview - Polyplus transfection
Protein & Virus production challenges - BPI Interview - Polyplus-transfection
Просмотров 2086 лет назад
Protein & Virus production challenges - BPI Interview - Polyplus-transfection
Genome editing - Polyplus-transfection
Просмотров 9446 лет назад
Genome editing - Polyplus-transfection
Trends in transfection - Polyplus-transfection
Просмотров 1,1 тыс.7 лет назад
Trends in transfection - Polyplus-transfection
siRNA transfection using INTERFERin® reagent
Просмотров 6 тыс.12 лет назад
siRNA transfection using INTERFERin® reagent
DNA transfection using jetPRIME reagent
Просмотров 13 тыс.13 лет назад
DNA transfection using jetPRIME reagent
Very useful information
A very dangerous technology. Scripture says, “For just as the days of Noah were, so will be the coming of the Son Of Man.”
Quite late to the party, but I had a question whenver you all end up seeing this. During the QA session, one of the presenters said to "avoid negatively charge plastics like polycarbonate", I was uner the impression that polycarbonate and PETG are both neutral... Or was he giving polycarbonate as an example of a plastic that is okay to use?
Mnmmm😊
i dont like this vide
I am not a scientist. Can you explain what you are doing and why?
So sick and demonic.
Thanks for the explications. Just one thing. You did not mention Magnetotransfection. Is it some kind of chemical transfection.
Covid vaccines
Remember all the hype for Ancestry? People gave their DNA and they told people likely scenario but used genetic material for testing. My theory.
No, that's exactly what it was. it's sole intention
All these medical words and terms. Its as if i'm hearing another language.
So do the research, and find out what the terminology means......
sick tunes bruh
So happy you enjoyed our very catchy music theme! Stay tuned for more epic videos 👨🔬
Thank you Alengo for these very clear explanations on jetCRISPR and the advantages of using protein delivery for genome editing with CRISPR technology. Marie Rouhier, Ozyme